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Seedlings of wheat (Triticum aestivum L. cv. TH-122) were exposed to excess of Cu, Zn, or Pb (5 mM) in solution via Agar-added culture media in order to investigate the dynamics of metal uptake from the culture media to individual wheat root tip cells. The experiments were performed by observation with a scanning electron microscope and selective probe analyses of the adsorbed metals on the root epidermis of individual cells in roots. Copper has been found to be more mobile than zinc or lead. The metal uptake from the culture media to the root tips was triggered at 1 h, and maximum uptake was found at 4-5 h. Copper and zinc take up are found to occur from the root epidermis to the cell wall. The accumulation of the four metals was found to be most intensive in the cauline cells and least in the quiescent center cells of the primary roots.24]\]. The relation between BMI and increased risk of recurrence in T1-2 N0 or T3 N0 cancer was also shown in Japan \[[@B21]\], Korea \[[@B22]\], Egypt \[[@B23]\] and USA \[[@B17]\].
Although TNM stage is reported to be the most significant factor for the recurrence of patients with invasive breast cancer \[[@B2],[@B4],[@B6],[@B7]\], in our study, the significant independent factors predicting recurrence was BMI regardless of stage. These findings suggest that BMI is a more useful and simple indicator of risk for recurrence of breast cancer than tumor staging in predicting
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Aspergillus japonicus lipase-mediated hydrolysis of a substrate molecule was found to be followed by activation of the substrate molecule through the formation of an enzyme-substrate co-complex. The process was inhibited by purification from A. japonicus culture filtrate of diolein, an inhibitor of the enzyme. Analysis of the hydrolytic reactions catalyzed by the lipase, in terms of substrate recognition sequence, indicated that the D-DEO sequence, which is common to all bioactive peptides, is the recognition site of the enzyme. Kinetic analyses using different lipase-substrate complexes revealed that the enzyme bound to the substrate co-complex preferentially in a cleavage-resistant reaction. The present results are the first experimental evid