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The physiological and ecological mechanisms by which allelopathic soil bacteria control plant growth are poorly understood. In this study, a molecular profiling approach was applied to study Pasteuria penetrans-soil interactions over a short time period at high cell densities. RNA transcriptome sequencing revealed a set of genes of known functions, which appeared to be differentially expressed in one of two pairwise comparisons between co-cultivated soil and P. penetrans cell suspensions. The experimental design enabled the analysis of long-term effects of population pressure. This allowed the identification of genes likely to be involved in the biocontrol and bioprotection functions of P. penetrans. These included systems involved in the production of antimicrobial secondary metabolites, which are responsible for the allelopathic activity of this bacterium. Genes of the diketopiperazine biosynthetic pathway were also identified. Transcripts for proteins of the type VI secretion system were also found. Genes coding for phospholipase D, which is known to be involved in P. penetrans swarming motility and the production of flagella, were differentially expressed during high cell densities. The proteome of P. penetrans cell suspensions of high cell densities exhibited a strong ribonuclease activity. This activity was localized to the periplasm and cytoplasm. The differential expression of some of these putative biocontrol and bioprotection genes in response to the population pressure reflects the importance of this population density effect in the allelopathic interactions of P. penetrans.Museum of Science Tokyo

The is a science museum situated in Shinjuku, Tokyo, Japan. It is one of the four museums under the authority of the Science and Technology Basic Research Organization (STart). It is located next to Iidabashi Station in Chiyoda-ku, Tokyo, near Tokyo Metropolitan Government Building, Tokyo Skytree, and Tokyo Metropolitan Government Building.

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