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The second group consisted of the Beylerbey of the Capital, who though.Regulation of expression of the early genes of M herpesvirus 6.
The early genes of human herpesvirus 6 (HHV-6) were mapped to two early regions and the major immediate-early (IE) gene had similarity to other human herpesviruses. Transcription of the major IE gene was present in 2 hours postinfection, whereas the secondary immediate-early (IE) genes were transcribed at 4 hours postinfection. The eight IE proteins had molecular weights of 79 to 90 kDa. In vitro-synthesized IE proteins initiated membrane-bound polyadenylated RNA in 2 hours postinfection. Substitutions in the codons of the IE gene prevented synthesis of the IE protein. Recombinant viruses that were able to express the IE gene were able to initiate transcripts that coded for the upstream early proteins, including the IE gene. The IE protein could be detected as immunoreactive products in infected cells. Plasmid-transfected cells were infected with recombinant virus, and proteins encoded by the recombinant IE gene were detectable. The presence of IE proteins was shown by immunofluorescent microscopy. Plasmid transfections of untransformed and SV40-transformed cells showed that the IE gene was actively transcribed during the latent phase of infection and was continuously transcribed during reactivation. The early gene transcription and protein production were shown to occur during lytic and latent infection in a human cell line, and the known IE proteins of HHV-6 were expressed during lytic infection in this cell line. Regulation of the expression of the IE gene during latency is a useful model system for studies of early gene regulation in alpha-herpesviruses.Q:
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